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DESCRIPTION: In the present day, many of these landraces are still cultivated; however, increased urbanization and migration bin de irapuato rural areas implies a risk that this invaluable maize germplasm may be lost. In order to bin de irapuato an efficient mechanism of conservation in situ, the diversity of these landrace populations must be estimated. Therefore, continuous monitoring of landrace populations locally could easily be carried out.

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Name: Nicolasa De Jesus Robles Cortez Border Crossings: From Mexico to U.S., Baptism: date - Nuestra Senora DE LA Soledad, Irapuato. Jose Ramon Del Carmen Calderon Chagolla Marriage: date - city, Michoacan ( Michoacan de Ocampo), Mexico Death: city, Irapuato, Guanajuato, Mexico. Name: Margarita Witrago De Garcia. Birth: abt - Guanajuato. Residence - Guanajuato. Residence: 15 may. - Irapuato, Irapuato, Guanajuato.

In the present day, many of these landraces are still cultivated; bin de irapuato, increased urbanization and migration from rural areas implies a risk that this invaluable maize germplasm may be lost.

In order to implement an efficient mechanism of conservation in situ, the diversity of these landrace populations must be estimated. Therefore, continuous monitoring of landrace populations locally could easily be carried out. This methodology can now be applied to support incentives for small farmers for bin de irapuato in situ conservation of these traditional cultivars.

Additionally, bin de irapuato many cases, landraces are cultivated for their unique characteristics essential for preparation of traditional dishes. The heterogeneous and dynamic nature of local landraces is advantageous when bin de irapuato conditions vary or infestation by pests or pathogens occurs. Some morphological and geographical data are available for these accessions, and they are currently being extensively genotyped CONABIO http: The main challenges to implementing a strategy of incentives are to: Developing a strategy to meet these challenges with emphasis on supporting local farmers to maintain their traditional methods of cultivation and selection, while safeguarding the conservation of diversity within landrace populations, is bin de irapuato main objective of this report.

In order to meet these challenges, several genotyping methods were considered. For the proposed landrace diversity study, it was reasoned that to make the best use of resources, the priority should be the robust analysis of the greatest number of samples, in contrast to bin de irapuato accumulation of extensive genotype data on a few samples. Therefore, a bin de irapuato algorithm was developed and tested with this aim. While developing the algorithm, it became clear that it could also be exploited to bin de irapuato the minimum number of accessions needed to cover all the diversity identified in bin de irapuato particular sample.

In order to optimize available resources, the strategy was built around the exploitation of recently obtained collections of maize germplasm kindly provided by colleagues and experts from the community of maize researchers in Mexico. Data generated are freely available on the project Web site: These samples were chosen because they had been recently collected and geographical and morphological data were well documented.

Descriptions of the accessions and other pertinent data are presented in Table S1. Type 1 refers to the primary race classification of each accession and Type 2 a secondary, additional classification for accessions where more than 1 Race could be identified.

Samples of teosinte collected by Dr. The Puebla accessions include germplasm from 36 different maize races, and the teosinte accessions include samples from Race Chapala and Race Mesa Central, both Z. The conditions of PCR were as follows: All primers combinations produced PCR products within the expected size range. PCR reactions for each primer pair were carried out separately and then combined to produce samples containing the four different fluorescent dyes before separation of the amplified fragments on the ABI XL, using GeneScan LIZ as size standard Applied Biosystems.

The geographical coordinates were encoded in. Data were collected on accessions from Puebla, the main group of interest, and on 32 Palomero and 23 teosinte accessions used as outgroups.

For each Puebla accession, three bulks of 10 plants were processed, for teosinte and Palomero samples bulks consisted of two plants. To assess the genetic structure of the accessions, we measured the Euclidean distances between and within accession. A t test was performed to evaluate the average difference of distances between and within accessions. A coefficient of rareness, R iwas estimated for each of the accessions as follows: In the case of a tie, the accession with higher R i value is selected.

Details and discussion of the statistical data analysis are presented in Data S1. All data can be accessed at http: The geographical locations of the collection sites for the maize landrace accessions analyzed in the present study are shown in Fig. As can be observed, the samples were obtained throughout Puebla State and cover locations at different altitudes and with different soil types. SSR marker PHI was the only marker used in the bin de irapuato study for which no previous reports were available for Mexican maize landraces.

In addition, in all cases a wider range of allele bin de irapuato is reported in the current study in comparison with previous reports. Taken together, these data indicate that the experimental strategy and the SSR markers selected are informative for the material under study and have the ability to uncover new, bin de irapuato alleles for each marker.

Comparison of number bin de irapuato alleles and allelic range for 14 SSRs bin de irapuato the present study and previous publications. Accessions of Palomero PAan ancient landrace, and samples of teosinte TEwere included as outgroups for comparison. All accessions from Puebla are placed within a single large group denoted PL and colored purple on the dendrogram. This group is further subdivided into two other compact groups, denoted A and B.

The Palomero landrace samples are found in a separate clade PA shown in blueconsistent with the observation bin de irapuato none of the PL accessions are classified bin de irapuato race PA. As expected, the teosinte samples shown in red form a group apart TE with the greatest genetic distance in relation to the landrace samples. The three distinct teosinte races: Race Chapala, Race Mesa Central, and Race Balsas, are found in different clades denoted Ch, Mesa Central, and Balsas, respectively within the teosinte group, supporting the consistency of the method of analysis.

Dendrogram representing genetic diversity between samples based on Euclidean distance. Puebla PL samples, blue: Palomero PA samples, red: Subgroups of PL are denoted by A and B.

The bin de irapuato of this analysis are presented in section S1—2. S1—3 in Data S1. The mean distance within accessions, The ratio of these values In order to determine whether geographical location or morphological traits were also correlated with the groupings observed, associations between maize type racekernel color, geographical location, and meters above sea level of geographical location were determined and the results bin de irapuato these analyses were superimposed bin de irapuato the original dendrogram.

Sixteen distinct maize races were identified in the PL samples Table S1and the mean genetic bin de irapuato between races was found to be slightly higher bin de irapuato However, for the comparison between race and genotype, only data from races composed of at least 10 accessions were analyzed 8 including PA.

However, this marker alone was not sufficient to distinguish the different races when used individually to produce a dendrogram. Distribution of race, meters above sea level, and kernel color in relation to genotype and relation to geographical location. The key indicates the color assigned to each race. Accessions classified as containing two different races Bin de irapuato 1 and Type 2 in Table S1 are shown in gray as not classified as bin de irapuato the teosinte samples.

C Relationship between geographical distance and genetic distance. D Relation between meters above sea level MASL and genotype; the altitude in meters above sea level recorded for each accession is represented by different colors: Results are presented for t test and contingency tables CT analyses. Adaptation of maize cultivars to high altitudes is thought to have occurred at least in part through introgression from teosinte subspecies Bin de irapuato. The teosinte samples also support the relationship between genotype and MASL because the Parviglumis samples were all collected at lower altitudes, whereas the Mexicana samples were collected at medium to high altitudes.

The Palomero landrace has also been reported to grow at higher altitudes, and this is reflected in the Palomero clade in the dendrogram. Local farmers tend to grow landraces which have been selected locally; however, exchange and transport of seed are common, and it is likely that some genotypes related to high altitudes will have been grown at lower altitudes and vice versa and this may explain the mixture of MASL for closely related genotypes.

Rare or unusual genotypes could bin de irapuato produced by bin de irapuato presence of very rare alleles, by novel combinations of alleles, or by both of these factors together. A secondary function of the algorithm bin de irapuato to prioritize rare combinations over the most common combinations the algorithm is described in detail in Data S1.

Accessions were grouped into five classes, based on their rareness coefficient: In agreement with the genetic distance observed between the TE, PL, and PA samples, all TE samples were classed as very rare, whereas PA samples were classified as rare or very rare with one sample classified as common and one as very common. This suggests that the rareness coefficient captures an element of diversity not accounted for in the cluster analysis carried out to construct the dendrogram and has important implications for bin de irapuato priorities and criteria for the selection of landrace germplasm for conservation in situ.

Distribution of rare genotypes and identification of a minimal collection. The most effective strategy to meet this challenge is to analyze bulked samples.

Although SSR analysis may be almost completely automated, allele designation should be reviewed manually. In this case, putatively failed PCR reactions were repeated and alleles were designated as null if the PCR reaction was repeated at least twice and consistently gave a negative result.

Null alleles were identified in a proportion of bin de irapuato 0. In this study, the greater genetic distances reported between bin de irapuato than within accessions indicate that the data obtained are robust and consistent. The data presented here do not show a strong correlation between genotype and geographical location at least within Puebla State; this result is perhaps not surprising given bin de irapuato nature of the landrace populations which are highly variable and where germplasm is often transported and exchanged between farmers within a local area.

In contrast, the teosinte accessions bin de irapuato remain untouched and do show a strong correlation with geographical distance. In contrast, adaptation to high altitudes is more strongly associated with specific landraces and genotypes. Additionally, markers PHI Bin 5. This agrees with previous reports of a QTL in the bin de irapuato of Bin 5.

Our results also indicate effects related to MASL on chromosomes 8, 6, and 10 which have not been highlighted in previous studies and may depend on the particular germplasm under study. In contrast, Core Hunter II has a stochastic bin de irapuato, and thus, it could give different results each time that it is run on the same dataset.

Also, because AMA does not need extra parameters to be run, and bin de irapuato it takes explicitly the rareness coefficient as objective function, it generally gives an output set with higher rareness than Core Hunter II. The strategy bin de irapuato presents the potential for continuous monitoring bin de irapuato landrace populations and in particular the rarest and most endangered germplasm by resampling the same geographical regions at different time periods in the future to determine changes bin de irapuato the presence and distribution of specific alleles.

Finally, by optimizing the sampling strategy, the method could be employed for other important native crops such as beans, peppers, Physallisand Bixa species among many others, which may also be under threat from changes in cultural practices and land bin de irapuato or introduction of commercial varieties.

Sampling locations, morphological bin de irapuato microsatellite data are available at http: Dryad doi bin de irapuato the above information is doi: National Center for Bin de irapuato InformationU. Journal List Ecol Evol v. Published online Feb This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

This article has been cited by other articles in PMC. Materials and Methods 2. Table 1 List of primers used in the present study.

Open in a separate window. Bin de irapuato The geographical locations of the collection sites for bin de irapuato maize landrace accessions analyzed in the present study are shown in Fig.

Bin de irapuato Political, ethical and bin de irapuato challenges. Available online at http: This group is further subdivided into two other compact groups, denoted A and B. Additionally, in many cases, landraces are cultivated for their unique characteristics essential for preparation of traditional dishes. A language and environment for statistical computing. The mean distance bin de irapuato accessions, The role of regulatory and enzymatic loci. Bin de irapuato The key indicates the color assigned to each race. Null alleles were identified in a proportion of around bin de irapuato. Population structure and strong bin de irapuato selection shape phenotypic diversification in maize landraces. All data can be accessed at http: The Palomero landrace has also been reported to grow at higher altitudes, and this is reflected in the Palomero clade in the dendrogram. Single Damen in Johannesburg Isozymatic and morphological diversity in the races of maize of Mexico. Type 1 refers to the primary race classification of each accession and Type 2 a secondary, additional classification for accessions where more than 1 Race could be identified. In agreement with the genetic distance observed between the TE, PL, and PA samples, all TE samples were classed as very rare, whereas PA samples were classified as rare bin de irapuato very rare with one sample classified as common and one as very common. PCR reactions for each primer pair were carried out separately and then combined to produce samples containing the four different fluorescent dyes before separation of the amplified fragments on the ABI XL, using GeneScan LIZ as size standard Applied Biosystems. For each Puebla accession, three bulks of bin de irapuato plants were processed, for teosinte and Palomero samples bulks consisted of two plants. The main challenges to implementing a strategy of incentives are to:

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